Extraction System Accessories
Sigma Aldrich Fine Chemicals Biosciences Protein Extraction Reagent
Protein Extraction Reagent Type 4 is a highly premixed prequalified and effective protein solubilization and extraction reagent composed of urea thiourea Trizma(R) and detergent C7BzO (C0856).
Sigma Aldrich Fine Chemicals Biosciences fatty Acid Extraction Kit
Lipids are structural components of cell membranes that play a critical role in gene transcription signaling and metabolism. Several lipid species exist in biological systems including phospholipids triglycerides and fatty acids. When extracting fatty acids at normal or high levels such as in food matrices use the MAK338 fatty acid extraction kit. This kit produces stronger signals. If in doubt about which kit to use use MAK338. When extracting fatty acids at low or very low levels such as in cellular matrices use the MAK174 fatty acid extraction kit. The folch method has been conventionally used to extract lipids containing fatty acids from biological samples using chloroform methanol and water to separate lipids from aqueous-soluble compounds. In this procedure lipids are retained in the lower chloroform layer whereas aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chl